DNA & RNA Extraction
AIOHRD offers high throughput extraction services for a variety of sample types. We produce high purity DNA and RNA for analysis from human, animal and plant samples. We provide customers with high quality genomic DNA preparations, which can be used for
- PCR
- Sanger Sequencing
- Library construction
- Next Generation Sequencing
- Genotyping
- Southern blotting
- Other molecular biology application
Sample collection
To maintain high-quality DNA yields, sample sets should be immediately preserved upon collection and properly shipped to AIOHRD. Sample sets received for genomic DNA preparation are logged, and stored at -70ºC until processing. After sample sets are processed, A260 and A280 readings are obtained and entered into the database to calculate the concentration and yield. A 1-µg aliquot of each sample is electrophoresed on a quality control check gel. Finished sample sets are frozen at -70ºC packaged with a data sheet and gel picture and returned to the customer.
Capability
We routinely offer the following DNA & RNA extraction from the following sources:
- Whole blood
- Saliva
- FTA paper
- Buccal Swabs
- Mammalian cell lines/primary cells (cell pellets)
- Plant and animal tissue
- Bacteria
Molecular biology is an integral tool for analysing treated tissues, building molecular probes and developing new models. Some of our expertise includes:
- Western, Northern and Southern blot analysis, immunoprecipitation and kinase assays
- Gene construction, transfection, and expression
- DNA sequencing and mutation detection
- Next Generation Sequencing
- cDNA synthesis, primer design, PCR, and RT-PCR
- Genotyping, DNA enzymology, plasmid DNA sub-cloning
- Microarray
For further information and prices please contact us or call +2348034387995 or send email to diagnostics@aiohrd.org
